Ed Asner
J. Jonah Jameson (voice)

Dorian Harewood
Tombstone (voice)

Christopher Daniel Barnes
Spider-Man/Peter Parker (voice)

Sara Ballantine
Mary Jane Watson (voice)

Leigh-Allyn Baker
Alisha Silvermain (voice)

Maxwell Caulfield
Alistair Smythe (voice)

Linda Gary
Tante May Parker (voice)

Gary Imhoff
Harry Osborn (voice)

Roscoe Lee Browne
Kingpin/Wilson Fisk (voice)